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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
The Spectronic 20 is a brand of single-beam spectrophotometer, designed to operate in the visible spectrum [1] across a wavelength range of 340 nm to 950 nm, with a spectral bandpass of 20 nm. [ 2 ] [ 3 ] It is designed for quantitative absorption measurement at single wavelengths. [ 1 ]
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
Ultraviolet–visible spectroscopy (UV–vis) can distinguish between enantiomers by showing a distinct Cotton effect for each isomer. UV–vis spectroscopy sees only chromophores, so other molecules must be prepared for analysis by chemical addition of a chromophore such as anthracene.
[17]: 18 Its designation as a "UV–Vis" spectrophotometer indicates its ability to measure light in both the visible and ultraviolet spectra. [ 29 ] The DU was the first commercially viable scientific instrument for measuring the amount of ultraviolet light absorbed by a substance.
In modern spectrographs in the UV, visible, and near-IR spectral ranges, the spectrum is generally given in the form of photon number per unit wavelength (nm or μm), wavenumber (μm −1, cm −1), frequency (THz), or energy (eV), with the units indicated by the abscissa.
Cary Model 14B Recording Spectrophotometer (front, details almost identical to Model 14) Cary Model 14 Recording Spectrophotometer (back, open) The Cary Model 14 UV-VIS Spectrophotometer was a double beam recording spectrophotometer designed to operate over the wide spectral range of ultraviolet, visible and near infrared wavelengths (UV/Vis/NIR).
The vast majority of liquid chromatographic systems are equipped with ultraviolet (UV) absorption detectors. The most common UV-Vis detectors used are variable wavelength detectors (VWD), photo diode array detectors (PDA), and diode array detectors (DAD). [4] Variable wavelength detectors decide in advance which wavelength is needed for the ...
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