Search results
Results from the WOW.Com Content Network
Gel permeation chromatography (GPC) [1] is a type of size-exclusion chromatography (SEC), that separates high molecular weight or colloidal analytes on the basis of size or diameter, typically in organic solvents. The technique is often used for the analysis of polymers.
Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers . [ 3 ]
Some of the most common methods for determining these parameters are colligative property measurements, static light scattering techniques, viscometry, and size exclusion chromatography. Gel permeation chromatography, a type of size exclusion chromatography, is an especially useful technique used to directly determine the molecular weight ...
Size-exclusion chromatography (SEC) is also known as gel permeation chromatography (GPC) or gel filtration chromatography and separates molecules according to their size (or more accurately according to their hydrodynamic diameter or hydrodynamic volume). Smaller molecules are able to enter the pores of the media and, therefore, molecules are ...
Gel permeation chromatography (also known as size-exclusion chromatography) Light scattering measurements such as dynamic light scattering; Direct measurement via mass spectrometry, using matrix-assisted laser desorption/ionization (MALDI) or electrospray ionization with tandem mass spectrometry (ESI-MS/MS)
Size-exclusion chromatography (SEC) [30] separates polymer molecules and biomolecules based on differences in their molecular size (actually by a particle's Stokes radius). The separation process is based on the ability of sample molecules to permeate through the pores of gel spheres, packed inside the column, and is dependent on the relative ...
This extraction may involve excision of the gel containing a band, or eluting the band directly off the gel as it runs off the end of the gel. In the context of a purification strategy, denaturing condition electrophoresis provides an improved resolution over size exclusion chromatography, but does not scale to large quantity of proteins in a ...
Reverse-phased size-exclusion chromatography (SEC) revealed pore size and pore size distribution of the particles and its dependence on temperature. Cyclohexanol acted as a successful porogen showing a dependent relationship of pore size to temperature. The use of toluene as a porogen gave results that were similar to unmodified macroporous ...