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  2. Auxotrophy - Wikipedia

    en.wikipedia.org/wiki/Auxotrophy

    In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...

  3. Canine cancer detection - Wikipedia

    en.wikipedia.org/wiki/Canine_cancer_detection

    Canine cancer detection is an approach to cancer screening that relies upon the claimed olfactory ability of dogs to detect, in urine or in breath, very low concentrations of the alkanes and aromatic compounds generated by malignant tumors.

  4. Selectable marker - Wikipedia

    en.wikipedia.org/wiki/Selectable_marker

    Selectable markers may serve as both positive and negative markers by conferring an advantage to the host under one condition, but inhibiting growth under a different condition. An example would be an enzyme that can complement an auxotrophy (positive selection) and be able to convert a chemical to a toxic compound (negative selection).

  5. Anti–Saccharomyces cerevisiae antibody - Wikipedia

    en.wikipedia.org/wiki/Anti–Saccharomyces...

    Mannans from other yeast, for example candida albicans, have found to cross react with ASCA which suggests that other yeast may induce ASCA associated diseases. ASCA are serological markers of candida albicans infections in humans and animals. [1] Mannan-binding lectin is a lectin produced by humans. In ASCA positive Crohn's disease the serum ...

  6. Likelihood ratios in diagnostic testing - Wikipedia

    en.wikipedia.org/wiki/Likelihood_ratios_in...

    A likelihood ratio of greater than 1 for a test in a population indicates that a positive test result is evidence that a condition is present. If the likelihood ratio for a test in a population is not clearly better than one, the test will not provide good evidence: the post-test probability will not be meaningfully different from the pretest ...

  7. Synthetic genetic array - Wikipedia

    en.wikipedia.org/wiki/Synthetic_genetic_array

    Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]

  8. Fungal prion - Wikipedia

    en.wikipedia.org/wiki/Fungal_prion

    Further investigation found that [PSI+] is the result of a self-propagating misfolded form of Sup35p (a 201 amino acid long protein), which is an important factor for translation termination during protein synthesis. [8] In [PSI+] yeast cells the Sup35 protein forms filamentous aggregates known as amyloid. The amyloid conformation is self ...

  9. Two-hybrid screening - Wikipedia

    en.wikipedia.org/wiki/Two-hybrid_screening

    This mutant yeast strain can be made to incorporate foreign DNA in the form of plasmids. In yeast two-hybrid screening, separate bait and prey plasmids are simultaneously introduced into the mutant yeast strain or a mating strategy is used to get both plasmids in one host cell. [9] The second high-throughput approach is the library screening ...

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