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Human iPS cells colonies. The spindle-shaped cells in the background are mouse fibroblast cells. Only those cells comprising the center colony are human iPS cells. Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell.
A stem cell line is a group of stem cells that is cultured in vitro and can be propagated indefinitely. Stem cell lines are derived from either animal or human tissues and come from one of three sources: embryonic stem cells, adult stem cells, or induced pluripotent stem cells. They are commonly used in research and regenerative medicine.
These pluripotent cells are called iPS (induced pluripotent stem) cells; they appeared with very low frequency. iPS cells can be selected by inserting the b-geo gene into the Fbx15 locus. The Fbx15 promoter is active in pluripotent stem cells which induce b-geo expression, which in turn gives rise to G418 resistance; this resistance helps us ...
James Alexander Thomson is an American developmental biologist best known for deriving the first human embryonic stem cell line in 1998 [1] and for deriving human induced pluripotent stem cells (iPS) in 2007. [2]
Using genetic reprogramming with protein transcription factors, pluripotent stem cells with ESC-like capabilities have been derived. [73] [74] [75] The first demonstration of induced pluripotent stem cells was conducted by Shinya Yamanaka and his colleagues at Kyoto University. [76]
For her post-doctoral work, she focused on cardiac stem cell biology and biomaterials. Here, her seminal work was to be the first scientist to demonstrate that induced pluripotent stem cells (iPSCs) differentiate towards the cardiovascular and hematopoietic lineages, showing that beating cardiomyocytes could be derived from iPSCs.
The European Bank for induced pluripotent Stem Cells (EBiSC) is a non-profit induced pluripotent stem cell (iPSC) biorepository and service provider with central facilities in Germany and the United Kingdom. EBiSC was set up between 2014 and 2017 by a consortium that represented researchers, clinicians and industry stakeholders. [1]
Naïve pluripotent stem cells (e.g. ESC) and primed pluripotent stem cells (e.g. EpiSC) not only sustain the ability to self-renew but also maintain the capacity to differentiate. [2] Since the cell status is primed to differentiate in EpiSCs, however, one copy of the X chromosome in XX cells (female cells) in EpiSCs is silenced (XaXi).
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