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A variety of different microorganisms form "spores" or "cysts", but the endospores of low G+C gram-positive bacteria are by far the most resistant to harsh conditions. [3] Some classes of bacteria can turn into exospores, also known as microbial cysts, instead of endospores. Exospores and endospores are two kinds of "hibernating" or dormant ...
Endospores can last for decades in multiple hard conditions, such as drying and freezing. This is because the DNA inside the endospore can survive over a long period. Most bacteria are unable to form endospores due to their high resistance, but some common species are the genera Bacillus ( over 100 species) and Clostridium (over 160 species). [2]
Endospores are bacterial survival structures that are highly resistant to many different types of chemical and environmental stresses and therefore enable the survival of bacteria in environments that would be lethal for these cells in their normal vegetative form.
Endospore-forming bacteria can cause disease; for example, anthrax can be contracted by the inhalation of Bacillus anthracis endospores, and contamination of deep puncture wounds with Clostridium tetani endospores causes tetanus, which, like botulism, is caused by a toxin released by the bacteria that grow from the spores. [101]
Acid fast bacteria:Red Non acid fast: Blue 5 Endospore (Dornor's method) Primary stain Malachite green heat fixed to penetrate spores; vegetative cells are counterstained with Safranin Detects the presence of endospores in six genera of bacteria Endospores: Green Vegetative cells: Red 6 Capsule A: Hiss method (Positive technique)
Clostridium endospores have a distinct bowling pin or bottle shape, distinguishing them from other bacterial endospores, which are usually ovoid in shape. [citation needed] The Schaeffer–Fulton stain (0.5% malachite green in water) can be used to distinguish endospores of Bacillus and Clostridium from other microorganisms. [12]
Fig1. The sporulation process of Bacillus subtilis. Bacillus subtilis is a rod-shaped, Gram-positive bacteria that is naturally found in soil and vegetation, and is known for its ability to form a small, tough, protective and metabolically dormant endospore.
Clostridium sporogenes is a species of Gram-positive bacteria that belongs to the genus Clostridium. Like other strains of Clostridium, it is an anaerobic, rod-shaped bacterium that produces oval, subterminal endospores [2] and is commonly found in soil. Unlike Clostridium botulinum, it does not produce the botulinum neurotoxins.