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Acinetobacter is a genus of Gram-negative bacteria belonging to the wider class of Gammaproteobacteria. Acinetobacter species are oxidase-negative, exhibit twitching motility, [7] and occur in pairs under magnification.
The traditional preparation caused mucous cells to swell during fixation which results in the serous cells being popped out of their alignment. After sectioning the serous cells resembled the common demilune shape, and were so named. [2] When the gland has this demilunar structure, it has mucoserous acini producing both serous and mucous ...
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology .
Acinetobacter baumannii is a typically short, almost round, rod-shaped (coccobacillus) Gram-negative bacterium.It is named after the bacteriologist Paul Baumann. [2] It can be an opportunistic pathogen in humans, affecting people with compromised immune systems, and is becoming increasingly important as a hospital-derived infection.
They contain serous acini, a grouping of serous cells that secrete serous fluid, isotonic with blood plasma, that contains enzymes such as alpha-amylase. Serous glands are most common in the parotid gland and lacrimal gland but are also present in the submandibular gland and, to a far lesser extent, the sublingual gland.
Mucoserous acini (singular acinus) or mixed acini are mainly present in submandibular and sublingual glands. They are formed by mucous cells with some serous cells interspersed each other. Both cells pour their secretion directly in the lumen. Layer of mucous cells and serous demilune of serous cells superficial to mucous cells
A pure culture is isolated and spread directly on a stainless steel or disposable target. The cells are lysed and overlaid with a matrix, which forms protein complexes with the bacterial proteins. The MALDI fires a laser and ionizes the protein complexes, which break off and travel up the vacuum where they are detected based on mass and charge.
The name VCC has also been used to describe the application of quantitative growth kinetics to enumerate bacteria in cell culture infection models. [3] Antimicrobial susceptibility testing (AST) can be done on 96-well plates by diluting the antimicrobial agent at varying concentrations in broth inoculated with bacteria and measuring the minimum ...