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Fine-needle aspiration (FNA) is a diagnostic procedure used to investigate lumps or masses. In this technique, a thin (23–25 gauge (0.52 to 0.64 mm outer diameter)), hollow needle is inserted into the mass for sampling of cells that, after being stained , are examined under a microscope ( biopsy ).
A fine needle aspirate can be done with simply a small bore needle and a small syringe (1 cc) that can generate rapid changes in suction pressure. Fine needle aspirate can be used to distinguish a cystic lesion from a lipoma. Both the surgeon and the pathologist must be familiar with the method of procuring, fixing, and reading of the slide.
Due to its short staining time, Diff-Quik stain is often used for initial screening of cytopathology specimens. This staining technique allows the cytotechnologist or pathologist to quickly assess the adequacy of the specimen, identify possible neoplastic or inflammatory changes, and decide whether or not additional staining is required. [4] [9 ...
The doctor will typically use a 22 or 27 gauge needle to aspirate out free fluid and cells. [12] It can be done in an outpatient setting and is associated with minimal pain. [12] However, in up to 30% of cases, pathological slides from fine-needle aspiration of breast lesions may be inconclusive, necessitating the need for further testing. [12]
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Aspiration sites are marked on the scrotal skin, 5 mm apart according to a template. The number of aspiration sites varies with testis size and ranges from 4 (to confirm obstruction) to 15 per testis (for nonobstructive azoospermia). FNA is performed with a sharp-beveled, 23-gauge, one-inch needle using the established suction cutting technique ...
In general, a fine-needle aspiration can be done anywhere it is safe to put a needle, including liver, lung, kidney, and superficial masses. Proper cytopathology technique takes time to master. Cytotechnologists and cytopathologists can assist clinicians by assisting with sample collection.
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