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Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
A cell line can be defined as a permanently established cell culture which will propagate forever. Investigators mostly get cell lines from other investigators or from cell banks (such as the American Type Culture Collection) , because its much easier than creating new one. In special cases, investigators are obligated to establish a cell line.
Obtaining a culture with a unified cell-cycle stage is useful for biological research where a particular stage in the cell cycle is desired (such as the culturing of parasitized cells [3]). Since cells are too small for certain research techniques, a synchronous culture can be treated as a single cell; the number of cells in the culture can be ...
Normally cell culture experiments are performed in a CO 2 incubator. Also perfusion culture experiments can be performed in such an atmosphere. However, a much better solution is the performance of perfusion culture experiments under atmospheric air on a laboratory table, since it facilitates the complete handling.
In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and/or increase the number of cells or microorganisms in the culture. [1]
The Image & Video Library Archived 2011-06-10 at the Wayback Machine of The American Society for Cell Biology contains many videos showing the cell division. The Cell Division of the Cell Image Library; Images : Calanthe discolor Lindl. – Flavon's Secret Flower Garden; Tyson's model of cell division and a Description on BioModels Database
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