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BOD test bottles at the laboratory of a wastewater treatment plant. Biochemical oxygen demand (also known as BOD or biological oxygen demand) is an analytical parameter representing the amount of dissolved oxygen (DO) consumed by aerobic bacteria growing on the organic material present in a water sample at a specific temperature over a specific time period.
Both have been widely adopted as a measure of pollution effect. The BOD test measures the oxygen demand of biodegradable pollutants whereas the COD test measures the oxygen demand of oxidizable pollutants. The so-called 5-day BOD measures the amount of oxygen consumed by biochemical oxidation of waste contaminants in a 5-day period.
For the Standard Methods 5210, [1] the BOD bottle “having 60 mL or greater capacity (300-mL)” is mentioned as one of the apparatus for the BOD test. A 60 mL BOD bottle is available and listed as "often convenient" by EPA (Environmental Protection Agency) Method 405.1. However, EPA Method 405.1 was written in 1974 and is no longer an EPA ...
Hach Company manufactures and distributes analytical instruments and reagents used to test the quality of water and other liquid solutions. Manufactured and distributed worldwide, Hach systems are designed to simplify analysis by offering on-line instrumentation, portable laboratory equipment, prepared reagents, easy-to-follow methods, and technical support.
The difference in DO and the dilution factor are used to calculated BOD 5. The resulting number (usually reported in parts per million or milligrams per liter) is useful in determining the relative organic strength of sewage or other polluted waters. The BOD 5 test is an example of analysis that determines classes of materials in a sample.
Water testing being conducted at a treatment facility in Broken Bow, Oklahoma. Water testing is a broad description for various procedures used to analyze water quality. Millions of water quality tests are carried out daily to fulfill regulatory requirements and to maintain safety. [1] Testing may be performed to evaluate:
The dip slide test consists of a sterile culture medium on a plastic carrier that is dipped into the liquid to be sampled. [3] The culture is then incubated, allowing for microbial growth. [ 2 ] Most Dip slides consist of 1 - 2 agars attached to a flexible plastic paddle, this allows full contact of the agar onto the desired area for testing. [ 4 ]
Dichromate, the oxidizing agent for COD determination, does not oxidize ammonia into nitrate, so nitrification is not included in the standard COD test. The International Organization for Standardization describes a standard method for measuring chemical oxygen demand in ISO 6060 . However, this ISO standard was withdrawn in 2024.
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