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The 3A assembly method is the most commonly used, as its compatible with assembly Standard 10, Silver standard as well as the Freiburg standard. This assembly method involves two BioBrick parts and a destination plasmid. The destination plasmid contains a toxic (lethal) gene, to ease the selection of a correctly assembled plasmid.
Assembly theory conceptualizes objects not as point particles, but as entities defined by their possible formation histories. [ 5] This allows objects to show evidence of selection, within well-defined boundaries of individuals or selected units. [ 5] Combinatorial objects are important in chemistry, biology and technology, in which most ...
Artificial gene synthesis, or simply gene synthesis, refers to a group of methods that are used in synthetic biology to construct and assemble genes from nucleotides de novo. Unlike DNA synthesis in living cells, artificial gene synthesis does not require template DNA, allowing virtually any DNA sequence to be synthesized in the laboratory.
In cell biology, the spindle apparatus is the cytoskeletal structure of eukaryotic cells that forms during cell division to separate sister chromatids between daughter cells. It is referred to as the mitotic spindle during mitosis, a process that produces genetically identical daughter cells, or the meiotic spindle during meiosis, a process ...
Kinesin is a protein functioning as a molecular biological machine. It uses protein domain dynamics on nanoscales. A protein complex or multiprotein complex is a group of two or more associated polypeptide chains. Protein complexes are distinct from multidomain enzymes, in which multiple catalytic domains are found in a single polypeptide chain.
Bottom–up and top–down design. Bottom–up and top–down are both strategies of information processing and ordering knowledge, used in a variety of fields including software, humanistic and scientific theories (see systemics ), and management and organization. In practice they can be seen as a style of thinking, teaching, or leadership.
Biological illustration. Illustration from the book Histoire naturelle by Louis Renard, published in Amsterdam in 1754. Biological illustration is the use of technical illustration to visually communicate the structure and specific details of biological subjects of study. This can be used to demonstrate anatomy, explain biological functions or ...
Graph Assembly: is based on Graph theory in computer science. The de Bruijn Graph is an example of this approach and utilizes k-mers to assemble a contiguous from reads. [15] Greedy Graph Assembly: this approach score each added read to the assembly and selects the highest possible score from the overlapping region.