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  2. DNA fragmentation - Wikipedia

    en.wikipedia.org/wiki/DNA_fragmentation

    Apoptotic DNA fragmentation is a natural fragmentation that cells perform in apoptosis (programmed cell death). DNA fragmentation is a biochemical hallmark of apoptosis.In dying cells, DNA is cleaved by an endonuclease that fragments the chromatin into nucleosomal units, which are multiples of about 180-bp oligomers and appear as a DNA ladder when run on an agarose gel. [8]

  3. TUNEL assay - Wikipedia

    en.wikipedia.org/wiki/TUNEL_assay

    TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells. [3] The assay relies on the use of terminal deoxynucleotidyl transferase (TdT), an enzyme that catalyzes attachment of deoxynucleotides, tagged with a fluorochrome or another ...

  4. Comet assay - Wikipedia

    en.wikipedia.org/wiki/Comet_assay

    These include genotoxicity testing, human biomonitoring and molecular epidemiology, ecogenotoxicology, as well as fundamental research in DNA damage and repair. [15] For example, Swain and Rao, using the comet assay [ 16 ] reported marked increases in several types of DNA damages in rat brain neurons and astrocytes during aging , including ...

  5. Apoptotic DNA fragmentation - Wikipedia

    en.wikipedia.org/wiki/Apoptotic_DNA_fragmentation

    The apoptotic DNA fragmentation is being used as a marker of apoptosis and for identification of apoptotic cells either via the DNA laddering assay, [2] the TUNEL assay, [3] [4] or the by detection of cells with fractional DNA content ("sub G 1 cells") on DNA content frequency histograms e.g. as in the Nicoletti assay.

  6. Sperm Chromatin Structure Assay - Wikipedia

    en.wikipedia.org/wiki/Sperm_Chromatin_Structure...

    Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA fragmentation. [1] First described by Evenson in 1980, the assay is a flow cytometric test that detects the vulnerability of sperm DNA to acid-induced denaturation DNA in situ. [2]

  7. EPIC-Seq - Wikipedia

    en.wikipedia.org/wiki/EPIC-Seq

    Circulating tumour DNA (ctDNA) molecules are tumour-derived cell-free DNA (cfDNA) circulating in the bloodstream and are not associated with cells. CtDNA primarily arises from chromatin fragmentation accompanying tumour cell death [12] and can be extracted by liquid biopsy. [13]

  8. Real-Life Stories of Sometimes-Shocking Home DNA Test Results

    www.aol.com/real-life-stories-sometimes-shocking...

    When Carl-Eric Benzinger took a 23andMe DNA test, the results revealed that he had a niece in Hawaii, according to WYPR in Baltimore. Carl-Eric reached out to his brother, Robert Benzinger, and ...

  9. DNA laddering - Wikipedia

    en.wikipedia.org/wiki/DNA_laddering

    DNA laddering (left) visualised in an agarose gel by ethidium bromide staining. A 1 kb marker (middle) and control DNA (right) are included.. DNA laddering is a feature that can be observed when DNA fragments, resulting from Apoptosis DNA fragmentation are visualized after separation by gel electrophoresis the first described in 1980 by Andrew Wyllie at the University Edinburgh medical school ...

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