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The test uses the principles of gel electrophoresis to separate out the various types of hemoglobin and is a type of native gel electrophoresis.After the sample has been treated to release the hemoglobin from the red cells, it is introduced into a porous gel (usually made of agarose or cellulose acetate) and subjected to an electrical field, most commonly in an alkaline medium.
Reference ranges (reference intervals) for blood tests are sets of values used by a health professional to interpret a set of medical test results from blood samples. Reference ranges for blood tests are studied within the field of clinical chemistry (also known as "clinical biochemistry", "chemical pathology" or "pure blood chemistry"), the ...
Hemoglobin. The mean corpuscular hemoglobin concentration (MCHC) is a measure of the concentration of hemoglobin in a given volume of packed red blood cell. It is calculated by dividing the hemoglobin by the hematocrit. Reference ranges for blood tests are 32 to 36 g/dL (320 to 360g/L), [1] or between 4.81 and 5.58 mmol/L.
So, in a person whose reticulocyte percentage is 5%, hemoglobin 7.5 g/dL, hematocrit 25%, the RPI would be: R P I = C o r r e c t e d R e t i c P e r c e n t a g e M a t u r a t i o n C o r r e c t i o n {\displaystyle RPI={CorrectedReticPercentage \over MaturationCorrection}} → RPI = 5 ∗ 25 45 2 = {\displaystyle ={{5*{25 \over 45}} \over 2 ...
MCH=(Hb*10)/RBC (in millions) [3] A normal MCH value in humans is 27 to 33 picograms (pg)/cell. The amount of hemoglobin per RBC depends on hemoglobin synthesis and the size of the RBC. [4] The mass of the red cell is determined by the iron (as part of the hemoglobin molecule), thus MCH in picograms is roughly the mass of one red cell.
The widely used [21] rule calculating normal maximum ESR values in adults (98% confidence limit) is given by a formula devised in 1983 from a study of ≈1000 individuals over the age of 20: [22] The normal values of ESR in men is age (in years) divided by 2; for women, the normal value is age (in years) plus 10, divided by 2.
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A complete blood count (CBC), also known as a full blood count (FBC), is a set of medical laboratory tests that provide information about the cells in a person's blood.The CBC indicates the counts of white blood cells, red blood cells and platelets, the concentration of hemoglobin, and the hematocrit (the volume percentage of red blood cells).