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BOD Bottle BOD test bottles at the laboratory of a wastewater treatment plant. BOD Bottle or an incubation bottle is a main apparatus used for the Biological Oxygen Demand (BOD) test. During the five-day BOD or BOD5 test process, the BOD bottle is used for incubating diluted samples under the 20 °C or 68 °F of temperature.
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BOD test bottles at the laboratory of a wastewater treatment plant. Biochemical oxygen demand (also known as BOD or biological oxygen demand) is an analytical parameter representing the amount of dissolved oxygen (DO) consumed by aerobic bacteria growing on the organic material present in a water sample at a specific temperature over a specific time period.
24/40 is common for 250 mL or larger flasks, while smaller sizes such as 14/20 or 19/22 are used for smaller flasks. Because of the round bottom, cork rings are needed to keep the round bottom flasks upright. When in use, round-bottom flasks are commonly held at the neck by clamps on a stand.
The BOD test is not bottle dependent, many labs use a 500ml bottle for the samples under test if using a titration method for determination or as small as 50ml if using an electronic DO probe, but the size of the bottle is only a function of practicality and space.
Common cap sizes include 33-430 (33mm), 38-430 (38mm), and GL 45 (45mm). Caps range in size from narrow mouthed to wide mouthed and often a glass or plastic funnel is needed to properly fill a reagent bottle from a larger or equal sized container's mouth. Reagent bottle caps are commonly said to be "autoclavable".