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  2. Cell culture - Wikipedia

    en.wikipedia.org/wiki/Cell_culture

    Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...

  3. Shaf Keshavjee - Wikipedia

    en.wikipedia.org/wiki/Shaf_Keshavjee

    Keshavjee and his research team have developed a technique of lung preservation that can improve lung function after its transplantation. The solution is made from low-potassium dextran and is used in the Ex Vivo. [citation needed] The LPD solution is applied in both the Toronto lung transplant program and in clinical programs around the world ...

  4. Precision cut lung slices - Wikipedia

    en.wikipedia.org/wiki/Precision_cut_lung_slices

    Start by carefully selecting lung tissue from the desired species, such as rodents or humans, ensuring the tissue is of high quality and health. Tissue Embedding To facilitate slicing and maintain tissue structure, the lung tissue is typically embedded in a suitable medium, such as agarose or gelatin, into the specimen holder of the vibratome.

  5. Organ-on-a-chip - Wikipedia

    en.wikipedia.org/wiki/Organ-on-a-chip

    Most organ-on-a-chip models today only culture one cell type, so even though they may be valid models for studying whole organ functions, the systemic effect of a drug on the human body is not verified. In particular, an integrated cell culture analog (μCCA) was developed and included lung cells, drug-metabolizing liver and fat cells.

  6. Organ culture - Wikipedia

    en.wikipedia.org/wiki/Organ_culture

    Organ culture is the cultivation of either whole organs or parts of organs in vitro. [1] It is a development from tissue culture methods of research, as the use of the actual in vitro organ itself allows for more accurate modelling of the functions of an organ in various states and conditions.

  7. Ex vivo lung perfusion - Wikipedia

    en.wikipedia.org/wiki/Ex_vivo_lung_perfusion

    Ex vivo lung perfusion, EVLP, is a form of machine perfusion aimed at sustaining the active aerobic cellular metabolism of donor lungs outside the donor's body prior to lung transplantation. This medical preservation technique typically occurs within a specialised machine engineered to mimic the conditions of the natural circulatory system .

  8. Tissue engineering - Wikipedia

    en.wikipedia.org/wiki/Tissue_engineering

    Micro-mass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. A commonly applied definition of tissue engineering, as stated by Langer [3] and Vacanti, [4] is "an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve [Biological tissue] function or a ...

  9. Lung-on-a-chip - Wikipedia

    en.wikipedia.org/wiki/Lung-on-a-chip

    Schematic drawing of a lung-on-a-chip. The membrane in the middle can be stretched by vacuum in the two side chambers. Lung-on-a-chip (LoC), also known as Lung Chips, are micro- and millifluidic organ-on-a-chip devices designed to replicate the structure and function of the human lung, mimicking the breathing motions and fluid dynamics that occur during inhalation and exhalation. [1]

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