Search results
Results from the WOW.Com Content Network
The ligase chain reaction (LCR) is a method of DNA amplification. The ligase chain reaction (LCR) is an amplification process that differs from polymerase chain reaction (PCR) in that it involves a thermostable ligase to join two probes or other molecules together which can then be amplified by standard PCR cycling. [ 1 ]
An amplicon sequence template that has been prepared for amplification. The target sequence to be amplified is colored green. In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events.
Liquid junction potential (shortly LJP) occurs when two solutions of electrolytes of different concentrations are in contact with each other. The more concentrated solution will have a tendency to diffuse into the comparatively less concentrated one.
MLPA facilitates the amplification and detection of multiple targets with a single primer pair. In a standard multiplex PCR reaction, each fragment needs a unique amplifying primer pair. These primers being present in a large quantity result in various problems such as dimerization and false priming. With MLPA, amplification of probes can be ...
It can be calculated using the equation Đ M = M w /M n, where M w is the weight-average molar mass and M n is the number-average molar mass. It can also be calculated according to degree of polymerization, where Đ X = X w /X n, where X w is the weight-average degree of polymerization and X n is the number-average
While the updated calculation has a lower impact than the original one, “this study just reveals one of the ways we’re exposed to flame retardants in our daily life,” Liu says. “We’re ...
Another example is the unimolecular nucleophilic substitution (S N 1) reaction in organic chemistry, where it is the first, rate-determining step that is unimolecular. A specific case is the basic hydrolysis of tert-butyl bromide (t-C 4 H 9 Br) by aqueous sodium hydroxide. The mechanism has two steps (where R denotes the tert-butyl radical t-C ...
Diet can help with constipation, eating more fiber and fewer high-fat foods. These are the most effective foods at relieving constipation, a dietitian says.