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Experiments on Plant Hybridization" (German: Versuche über Pflanzen-Hybriden) is a seminal paper written in 1865 and published in 1866 [1] [2] by Gregor Mendel, an Augustinian friar considered to be the founder of modern genetics. The paper was the result after years spent studying genetic traits in Pisum sativum, the pea plant.
The first uses of test crosses were in Gregor Mendel’s experiments in plant hybridization.While studying the inheritance of dominant and recessive traits in pea plants, he explains that the “signification” (now termed zygosity) of an individual for a dominant trait is determined by the expression patterns of the following generation.
Mendel cross-bred tall and dwarf pea plants, green and yellow peas, purple and white flowers, wrinkled and smooth peas, and a few other traits. He then observed the resulting offspring. In each of these cases, one trait is dominant and all the offspring, or Filial-1 (abbreviated F 1) generation, showed the
(green pod color is dominant over yellow for pea pods [1] in contrast to pea seeds, where yellow cotyledon color is dominant over green [2]). Punnett squares for each combination of parents' colour vision status giving probabilities of their offsprings' status, each cell having 25% probability in theory.
[29] [30] [31] This study showed that, when true-breeding different varieties were crossed to each other (e.g., tall plants fertilized by short plants), in the second generation, one in four pea plants had purebred recessive traits, two out of four were hybrids, and one out of four were purebred dominant.
In a dominant-recessive inheritance, an average of 25% are homozygous with the dominant trait, 50% are heterozygous showing the dominant trait in the phenotype (genetic carriers), 25% are homozygous with the recessive trait and therefore express the recessive trait in the phenotype. The genotypic ratio is 1: 2 : 1, and the phenotypic ratio is 3: 1.
The garden pea was chosen as an experimental organism because many varieties were available that bred true for qualitative traits and their pollination could be manipulated. The seven variable characteristics Mendel investigated in pea plants were. [5] seed texture (round vs wrinkled) seed color (yellow vs green) flower color (white vs purple)
An allele is said to be dominant if it is always expressed in the appearance of an organism (phenotype) provided that at least one copy of it is present. For example, in peas the allele for green pods, G, is dominant to that for yellow pods, g. Thus pea plants with the pair of alleles either GG (homozygote) or Gg (heterozygote) will have green ...