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pH can be measured using indicators, which change color depending on the pH of the solution they are in. By comparing the color of a test solution to a standard color chart, the pH can be estimated to the nearest whole number. For more precise measurements, the color can be measured using a colorimeter or spectrophotometer.
The isoelectric point (pI, pH(I), IEP), is the pH at which a molecule carries no net electrical charge or is electrically neutral in the statistical mean. The standard nomenclature to represent the isoelectric point is pH(I). [1] However, pI is also used. [2] For brevity, this article uses pI.
Intracellular pH (pH i) is the measure of the acidity or basicity (i.e., pH) of intracellular fluid. The pH i plays a critical role in membrane transport and other intracellular processes. In an environment with the improper pH i , biological cells may have compromised function.
Acid–base homeostasis is the homeostatic regulation of the pH of the body's extracellular fluid (ECF). [1] The proper balance between the acids and bases (i.e. the pH) in the ECF is crucial for the normal physiology of the body—and for cellular metabolism. [1]
In and of themselves, pH indicators are usually weak acids or weak bases. The general reaction scheme of acidic pH indicators in aqueous solutions can be formulated as: HInd (aq) + H 2 O (l) ⇌ H 3 O + (aq) + Ind − (aq) where, "HInd" is the acidic form and "Ind −" is the conjugate base of the indicator. Vice versa for basic pH indicators ...
Phenol red, 40 μM: colors in cell culture medium at a pH range from 6.0 to 8.0. Most living tissues prosper at a near-neutral pH—that is, a pH close to 7. The pH of blood ranges from 7.35 to 7.45, for instance. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. A small amount of ...
Alkaliphiles are a class of extremophilic microbes capable of survival in alkaline (pH roughly 8.5–11) environments, growing optimally around a pH of 10. These bacteria can be further categorized as obligate alkaliphiles (those that require high pH to survive), facultative alkaliphiles (those able to survive in high pH, but also grow under normal conditions) and haloalkaliphiles (those that ...
The following other wikis use this file: Usage on bg.wikipedia.org Водороден показател; Usage on en.wikibooks.org Principles of Biochemistry/Water: The solvent of the cell