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For a standard agarose gel electrophoresis, 0.7% gel concentration gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel concentration gives good resolution for small 0.2–1kb fragments. Up to 3% gel concentration can be used for separating very tiny fragments but a vertical polyacrylamide gel would be more ...
Postulated migration of proteins in a Laemmli gel system A: Stacking gel, B: Resolving gel, o: sample application c: discontinuities in the buffer and electrophoretic matrix. Most protein separations are performed using a "discontinuous" (or DISC) buffer system that significantly enhances the sharpness of the bands within the gel. During ...
Agarose gel has large pore size and good gel strength, making it suitable as an anticonvection medium for the electrophoresis of DNA and large protein molecules. The pore size of a 1% gel has been estimated from 100 nm to 200–500 nm, [4] [5] and its gel strength allows gels as dilute as 0.15% to form a slab for gel electrophoresis. [6]
Null hypothesis (H 0) Positive data: Data that enable the investigator to reject a null hypothesis. Alternative hypothesis (H 1) Suppose the data can be realized from an N(0,1) distribution. For example, with a chosen significance level α = 0.05, from the Z-table, a one-tailed critical value of approximately 1.645 can be obtained.
If that likelihood is sufficiently small (e.g., less than 1%), the existence of a relation may be assumed. Otherwise, any observed effect may be due to pure chance. In statistical hypothesis testing, two hypotheses are compared. These are called the null hypothesis and the alternative hypothesis. The null hypothesis is the hypothesis that ...
Riemann knew that the non-trivial zeros of the zeta function were symmetrically distributed about the line s = 1/2 + it, and he knew that all of its non-trivial zeros must lie in the range 0 ≤ Re(s) ≤ 1. He checked that a few of the zeros lay on the critical line with real part 1/2 and suggested that they all do; this is the Riemann hypothesis.
Silica gel's high specific surface area—around 750–800 m 2 /g (230,000–240,000 sq ft/oz) [6] —allows it to adsorb water readily, making it useful as a desiccant (drying agent). Silica gel is often described as "absorbing" moisture, which may be appropriate when the gel's microscopic structure is ignored, as in silica gel packs or other ...
An upturned vial of hair gel Silica gel. A gel is a semi-solid that can have properties ranging from soft and weak to hard and tough. [1] [2] Gels are defined as a substantially dilute cross-linked system, which exhibits no flow when in the steady state, although the liquid phase may still diffuse through this system. [3] IUPAC definition for a gel