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Filtration rate is a function of the viscosity of the feed fluid. From equation (1), the relationship of the filtration rate is inversely proportional to the viscosity. Increasing viscosity means adding resistance to the fluid flow, which complicates separation of the fluids from the slurry. Consequently, the throughput of the pusher is de ...
By 1959, Millipore made porous membrane filters of cellulose esters or other materials which resembled paper in sheet form, and were brittle when dry but friable when wet. [5] Filters consisted of nitrocellulose or polycarbonate membrane nucleopore filters ranging from pore size of 0.2 μm ( micrometer ) to 20 μm.
Filter the mixture through a filter disk made of nitrocellulose. Proteins bind to nitrocellulose, but DNA does not. Any DNA that is retained on the filter is there because it is interacting with the protein. Dry the filters and count. Measure the off-rate: Take the filter with DNA-protein complex bound on it; Wash the filter with buffer
Nitrocellulose (also known as cellulose nitrate, flash paper, flash cotton, guncotton, pyroxylin and flash string, depending on form) is a highly flammable compound formed by nitrating cellulose through exposure to a mixture of nitric acid and sulfuric acid.
In addition, compared to the nitrocellulose membrane which requires a high ionic strength buffer to bind the DNA fragments to the membrane, nylon charged membranes use buffers with very low ionic strength to transfer even small fragments of DNA of about 50 bp to the membrane, usually the DNA to be transferred is separated by polyacrylamide gel.
Paper-based microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that transport fluid from an inlet through the porous medium to a desired outlet or region of the device, by means of capillary action. [1]
The first symptoms of kidney failure are silent. Failing kidneys can’t remove extra fluid from the body, nor can they filter molecules like urea, which can be toxic in high dosages, from the blood.
Following electrophoresis, a standard tank or semi-dry blotting transfer system is set up. A stack is put together in the following order from cathode to anode: sponge | three sheets of filter paper soaked in transfer buffer | gel | PVDF or nitrocellulose membrane | three sheets of filter paper soaked in transfer buffer | sponge.
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