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RNA sequencing is a next-generation sequencing technology; as such it requires only a small amount of RNA and no previous knowledge of the genome. [3] It allows for both qualitative and quantitative analysis of RNA transcripts, the former allowing discovery of new transcripts and the latter a measure of relative quantities for transcripts in a ...
RNA-Seq is accomplished by reverse transcribing RNA in vitro and sequencing the resulting cDNAs. [10] Transcript abundance is derived from the number of counts from each transcript. The technique has therefore been heavily influenced by the development of high-throughput sequencing technologies.
RNA-Seq (named as an abbreviation of RNA sequencing) is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample, providing a snapshot of gene expression in the sample, also known as transcriptome.
Gene ontology terms describe gene functions and the relationships between those functions into three classes: Molecular function; Cellular component; Biological process; Gene Ontology (GO) term enrichment is a technique used to identify which GO terms are over-represented or under-represented in a given set of genes. In single-cell analysis ...
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [1] It is primarily used to measure the amount of a specific RNA.
RNA polymerase, assisted by one or more general transcription factors, then selects a transcription start site in the transcription bubble, binds to an initiating NTP and an extending NTP (or a short RNA primer and an extending NTP) complementary to the transcription start site sequence, and catalyzes bond formation to yield an initial RNA product.
Micrograph of gene transcription of ribosomal RNA illustrating the growing primary transcripts. A primary transcript is the single-stranded ribonucleic acid product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs.
The ENCyclOpedia of Rna Epitranscriptome (ENCORE) is an upgraded version of RMBase that a comprehensive epitranscriptome platform with tens of new software and tools, to decode the distribution pattern, metagene profile, biogenesis mechanisms, regulatory functions, interactome, evolutional conservation and novel reader proteins of more than 70 ...