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  2. Kovats retention index - Wikipedia

    en.wikipedia.org/wiki/Kovats_retention_index

    In gas chromatography, the Kovats retention index (shorter Kovats index, retention index; plural retention indices) is used to convert retention times into system-independent constants. The index is named after the Hungarian-born Swiss chemist Ervin Kováts , who outlined the concept in the 1950s while performing research into the composition ...

  3. List of boiling and freezing information of solvents - Wikipedia

    en.wikipedia.org/wiki/List_of_boiling_and...

    Upload file; Search. Search. Appearance. Donate; Create account; ... Solvent Density (g cm-3) Boiling point (°C) ... Toggle the table of contents.

  4. Resolution (chromatography) - Wikipedia

    en.wikipedia.org/wiki/Resolution_(chromatography)

    Example chromatogram showing signal as a function of retention time. In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1] [2] [3] [4]

  5. Retardation factor - Wikipedia

    en.wikipedia.org/wiki/Retardation_factor

    An R F value will always be in the range 0 to 1; if the substance moves, it can only move in the direction of the solvent flow, and cannot move faster than the solvent. For example, if particular substance in an unknown mixture travels 2.5 cm and the solvent front travels 5.0 cm, the retardation factor would be 0.50.

  6. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    A mobile phase in reversed-phase chromatograpy consists of mixtures of water or aqueous buffers, to which organic solvents are added, to elute analytes from a reversed-phase column in a selective manner. [7] [27] The added organic solvents must be miscible with water, and the two most common organic solvents used are acetonitrile and methanol.

  7. Gel permeation chromatography - Wikipedia

    en.wikipedia.org/wiki/Gel_permeation_chromatography

    Samples are dissolved in an appropriate solvent, in the case of GPC these tend to be organic solvents and after filtering the solution it is injected onto a column. The separation of multi-component mixture takes place in the column. The constant supply of fresh eluent to the column is accomplished by the use of a pump.

  8. Chromatography software - Wikipedia

    en.wikipedia.org/wiki/Chromatography_software

    Chromatography software is called also Chromatography Data System. [1] It is located in the data station of the modern liquid, gas or supercritical fluid chromatographic systems.

  9. Column chromatography - Wikipedia

    en.wikipedia.org/wiki/Column_chromatography

    The mobile phase or eluent is a solvent or a mixture of solvents used to move the compounds through the column. It is chosen so that the retention factor value of the compound of interest is roughly around 0.2 - 0.3 in order to minimize the time and the amount of eluent to run the chromatography. The eluent has also been chosen so that the ...