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The molar extinction coefficient of Hb has its highest absorption peak at 420 nm and a second peak at 580 nm. Its spectrum then gradually decreases as light wavelength increases. On the other hand, H b O 2 {\displaystyle HbO2} shows its highest absorption peak at 410 nm, and two secondary peaks at 550 nm and 600 nm.
The SI unit of molar absorption coefficient is the square metre per mole (m 2 /mol), but in practice, quantities are usually expressed in terms of M −1 ⋅cm −1 or L⋅mol −1 ⋅cm −1 (the latter two units are both equal to 0.1 m 2 /mol).
Hemoglobin in the blood carries oxygen from the respiratory organs (lungs or gills) to the other tissues of the body, where it releases the oxygen to enable aerobic respiration which powers an animal's metabolism. A healthy human has 12 to 20 grams of hemoglobin in every 100 mL of blood. Hemoglobin is a metalloprotein, a chromoprotein, and ...
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This quantity is called the extinction coefficient and denoted κ. In accordance with the ambiguity noted above , some authors use the complex conjugate definition, where the (still positive) extinction coefficient is minus the imaginary part of n _ {\displaystyle {\underline {n}}} .
Ellman's original 1959 publication estimated the molar extinction at 13,600 M −1 cm −1, and this value can be found in some modern applications of the method despite improved determinations. [6] Commercial DTNB may not be completely pure, so may require recrystallization to obtain completely accurate and reproducible results.
When an isosbestic plot is constructed by the superposition of the absorption spectra of two species (whether by using molar absorptivity for the representation, or by using absorbance and keeping the same molar concentration for both species), the isosbestic point corresponds to a wavelength at which these spectra cross each other.
Extinction coefficient refers to several different measures of the absorption of light in a medium: Attenuation coefficient , sometimes called "extinction coefficient" in meteorology or climatology Mass extinction coefficient , how strongly a substance absorbs light at a given wavelength, per mass density