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Mechanism of self-amplifying mRNA (saRNA) used for antigen production. The ORF encoding the antigen can also be substituted with a protein for use in protein replacement therapy. This sequence encodes the protein of interest, used as an antigen in the case of vaccines or for protein replacement therapies. The gene of interest replaces the viral ...
To identify an saRNA that can activate a gene of interest, several saRNAs need to be designed within a 1- to 2-kbp promoter region by following a set of rules [3] [4] and tested in cultured cells. In some reports, saRNAs are designed in such a way to target non-coding transcripts that overlap the promoter sequence of a protein coding gene.
Breads at a restaurant. This is a list of baked goods.Baked goods are foods made from dough or batter and cooked by baking, [1] a method of cooking food that uses prolonged dry heat, normally in an oven, but also in hot ashes, or on hot stones.
Place half the spaghetti in an even layer in a 13-by-9-inch baking dish. Using a rubber spatula, spread the cheese mixture evenly over the pasta. Add the remaining spaghetti in an even layer.
The same principle applies when baking soda is used to boil pretzels before baking. The pantry staple can also serve as a tenderizer for certain ingredients—like meat and even canned chickpeas ...
Transfer to an 8x8 baking dish. In the same bowl, add the remaining oats and brown sugar. Cut in the butter using a pastry cutter or by smooshing with your hands to form a crumbly mixture.
Each usually consists of minced pork, rice, onion, eggs, thyme, and dill rolled in a leaf, usually a cabbage leaf. The baking dish is lined with chopped cabbage and sauerkraut layered with bacon or pork belly and the cabbage rolls, then topped with more sauerkraut and dill sprigs. The cooking water is poured over the assembled tray, a mixture ...
This healthy dinner recipe is comfort food at its best. ... with sherry and the potatoes are mashed with olive oil in this healthy shepherd's pie recipe. To make individual pies, use six 10-ounce ...