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In nature complementarity is the base principle of DNA replication and transcription as it is a property shared between two DNA or RNA sequences, such that when they are aligned antiparallel to each other, the nucleotide bases at each position in the sequences will be complementary, much like looking in the mirror and seeing the reverse of ...
In genetics, complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both single-stranded and double-stranded forms and in both natural and engineered forms.
An inverted repeat (or IR) is a single stranded sequence of nucleotides followed downstream by its reverse complement. [1] The intervening sequence of nucleotides between the initial sequence and the reverse complement can be any length including zero. For example, 5'---TTACGnnnnnn CGTAA---3' is an inverted repeat sequence.
Because of the complementary nature of base-pairing between nucleic acid polymers, a double-stranded DNA molecule will be composed of two strands with sequences that are reverse complements of each other. To help molecular biologists specifically identify each strand individually, the two strands are usually differentiated as the "sense" strand ...
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [1] It is primarily used to measure the amount of a specific RNA.
(In each strand, tag α is the reverse complement of tag β and vice versa). The libraries containing duplex tags and Illumina adapters are sequenced using the Illumina TruSeq system. Reads that are originating from every single strand of DNA form a group of reads (tag families) that share the same tag.
Reverse transcription is part of the replication cycle of particular virus families, including retroviruses. It involves copying RNA into double-stranded complementary DNA (cDNA), using reverse transcriptase enzymes. In retroviruses, viral RNA is inserted into a host cell nucleus.
Since the two reverse complementary sequences will fold and base-pair with each other, the sequence of bases between them form a hairpin loop.This structure is thought to destabilize the binding of RNA polymerase enzyme to DNA (hence terminating transcription).