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  2. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and ...

  3. Boom method - Wikipedia

    en.wikipedia.org/wiki/Boom_method

    Nucleic acid extraction apparatus based on the Tajima pipette [14] [15] (see Fig. 2) are one of the most widespread instruments to perform the Boom method. [ 25 ] The Tajima pipette was invented by Hideji Tajima, [ 14 ] founder and president of Precision System Sciences (PSS) [ 25 ] Inc., a Japanese manufacturer of precision and measuring ...

  4. Nucleic acid methods - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_methods

    Upload file; Special pages ... Cite this page; Get shortened URL; Download QR code; Print/export Download as PDF; Printable version ... Protocols for Recombinant DNA ...

  5. Molecular paleontology - Wikipedia

    en.wikipedia.org/wiki/Molecular_paleontology

    Isolation and sequencing of nuclear DNA has also been accomplished from the Denisova finger bone. This specimen showed an unusual degree of DNA preservation and low level of contamination. They were able to achieve near-complete genomic sequencing, allowing a detailed comparison with Neanderthal and modern humans.

  6. Comparative genomic hybridization - Wikipedia

    en.wikipedia.org/wiki/Comparative_genomic...

    In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two ...

  7. Southern blot - Wikipedia

    en.wikipedia.org/wiki/Southern_blot

    DNA Isolation: The DNA to be studied is isolated from various tissues. The most suitable source of DNA is known as blood tissue. However, it can be isolated from different tissues (hair, semen, saliva, etc.). DNA digestion: Restriction endonucleases are used to cut high-molecular-weight DNA strands into smaller fragments. This is done by adding ...

  8. Plasmid preparation - Wikipedia

    en.wikipedia.org/wiki/Plasmid_preparation

    Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.

  9. Agarose gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Agarose_gel_electrophoresis

    Estimation of the DNA concentration by comparing the intensity of the nucleic acid band with the corresponding band of the size marker. [34] Analysis of products of a polymerase chain reaction (PCR), e.g., in molecular genetic diagnosis or genetic fingerprinting; Separation of DNA fragments for extraction and purification.