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For bright-field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin and India ink.The specimen, such as a wet bacterial culture spread on a glass slide, is mixed with the negative stain and allowed to dry.
India ink (British English: Indian ink; [1] also Chinese ink) is a simple black or coloured ink once widely used for writing and printing and now more commonly used for drawing and outlining, especially when inking comic books and comic strips. India ink is also used in medical applications.
India ink was invented in China, [11] [12] though materials were often traded from India, hence the name. [11] [12] The traditional Chinese method of making the ink was to grind a mixture of hide glue, carbon black, lampblack, and bone black pigment with a pestle and mortar, then pour it into a ceramic dish to dry. [11]
Top to bottom: blue Lamy T 10 proprietary ink cartridge and Z 27 and Z 28 ink converters. Fountain pens carry ink within the barrel, traditionally either inserted at one end in bulk with a syringe or eyedropper pipette, or through a mechanical filling system built into the pen (such as a piston or vacuum-pump mechanism).
Structure of a major component of the dye nigrosin. In staining dyes, nigrosin (CI 50415, Solvent black 5) is a mixture of black synthetic dyes made by heating a mixture of nitrobenzene, aniline, and hydrochloric acid in the presence of copper or iron. [1] Related to induline, it is a mixture of phenazine-based compounds.
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