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In diethyl ether, chlorophyll a has approximate absorbance maxima of 430 nm and 662 nm, while chlorophyll b has approximate maxima of 453 nm and 642 nm. [25] The absorption peaks of chlorophyll a are at 465 nm and 665 nm. Chlorophyll a fluoresces at 673 nm (maximum) and 726 nm.
Chlorophyll a absorbs light within the violet, blue and red wavelengths. Accessory photosynthetic pigments broaden the spectrum of light absorbed, increasing the range of wavelengths that can be used in photosynthesis. [5] The addition of chlorophyll b next to chlorophyll a extends the absorption spectrum.
Absorbance spectra of free chlorophyll a (blue) and b (red) in a solvent. The action spectra of chlorophyll molecules are slightly modified in vivo depending on specific pigment-protein interactions. An action spectrum is a graph of the rate of biological effectiveness plotted against wavelength of light. [1]
The absorption spectrum of both the chlorophyll a and the chlorophyll b pigments. The use of both together enhances the size of the absorption of light for producing energy. Chlorophyll b is a form of chlorophyll. Chlorophyll b helps in photosynthesis by absorbing light energy.
Top: Absorption spectra for chlorophyll-A, chlorophyll-B, and carotenoids extracted in a solution. Bottom: PAR action spectrum (oxygen evolution per incident photon) of an isolated chloroplast. Chlorophyll, the most abundant plant pigment, is most efficient in capturing red and blue light.
Chlorophyll a is the most common of the six, present in every plant that performs photosynthesis. Each pigment absorbs light more efficiently in a different part of the electromagnetic spectrum. Chlorophyll a absorbs well in the ranges of 400–450 nm and at 650–700 nm; chlorophyll b at 450–500 nm and at 600–650 nm. Xanthophyll absorbs ...
The nature of the central metal can also influence the absorption spectrum of the metal-macrocycle complex or properties such as excited state lifetime. [ 4 ] [ 5 ] [ 6 ] The tetrapyrrole moiety in organic compounds which is not macrocyclic but still has a conjugated pi-bond system still acts as a chromophore.
The different chlorophyll and non-chlorophyll pigments associated with the photosystems all have different absorption spectra, either because the spectra of the different chlorophyll pigments are modified by their local protein environment or because the accessory pigments have intrinsic structural differences.