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This process recovers the antigens masked by formalin fixation. As a result, it enables the successful application of immunohistochemistry on formalin fixed paraffin embedded tissue sections. Without antigen retrieval, most immunostains on formalin fixed paraffin embedded tissue sections show no staining.
The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer
It guides the treatment for the sub-chronic and chronic management of soft tissue injuries. [12] There is also evidence that points towards using heat to treat acute and soft tissue injuries. Heat has the opposite effect of ice, which restricts blood flow and slows the healing process. The use of heat will open up the blood vessels in the ...
Paraffin wax may also be too soft in relation to the tissue, the heat of the melted wax may alter the tissue in undesirable ways, or the dehydrating or clearing chemicals may harm the tissue. [12] Alternatives to paraffin wax include, epoxy , acrylic , agar , gelatin , celloidin , and other types of waxes.
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
Myofascial release (MFR, self-myofascial release) is an alternative medicine therapy claimed to be useful for treating skeletal muscle immobility and pain by relaxing contracted muscles, improving blood and lymphatic circulation and stimulating the stretch reflex in muscles.
Post-traumatic arthritis is a form of osteoarthritis and the former can occur after the latter. However, post-traumatic arthritis can also occur after the development of chronic inflammatory arthritis. Generally, post-traumatic arthritis is classified in two groups: post-traumatic osteoarthritis and post-traumatic inflammatory arthritis. [2]
Pain, swelling, or stiffness in one or more joints is commonly present in psoriatic arthritis. [5] Psoriatic arthritis is inflammatory, and affected joints are generally red or warm to the touch. [5] Asymmetrical oligoarthritis, defined as inflammation affecting two to four joints during the first six months of disease, is present in 70% of ...
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