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A Basophil granulocyte stains dark purple upon H&E staining. Basophilic is a technical term used by pathologists. It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye. The most common such dye is haematoxylin.
Basophils are a type of white blood cell. Basophils are the least common type of granulocyte , representing about 0.5% to 1% of circulating white blood cells . [ 1 ] They are the largest type of granulocyte.
Hematoxylin principally colors the nuclei of cells blue or dark-purple, [6] [15] [14] along with a few other tissues, such as keratohyalin granules and calcified material. Eosin stains the cytoplasm and some other structures including extracellular matrix such as collagen [ 5 ] [ 7 ] [ 14 ] in up to five shades of pink. [ 8 ]
Basophilia is the condition of having greater than 200 basophils/μL in the venous blood. [1] Basophils are the least numerous of the myelogenous cells, and it is rare for their numbers to be abnormally high without changes to other blood components.
Other widely used metachromatic stains include the family of Romanowsky stains that also contain thiazine dyes: the white cell nucleus stains purple, basophil granules intense magenta, whilst the cytoplasms (of mononuclear cells) stains blue, which is called the Romanowsky effect. The absence of color change in staining is named orthochromasia.
Blue nucleus, pink cytoplasm, violet granules Eosinophils: Blue nucleus, blue cytoplasm, red granules Basophils: Purple/dark blue nucleus, violet granules Monocyte: Violet nucleus, light blue cytoplasm Bacteria and fungi: Dark blue Cytoplasm, collagen and muscle: Various shades of pink, orange, yellow and blue [11] Spermatozoa: Light blue ...
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil because it is basophilic (readily takes up bases), and typically stains a relatively deep blue or purple. [1] These basophils are further classified by the hormones they produce.
Volutin granules are an intracytoplasmic storage form of complexed inorganic polyphosphate, [1] the production of which is used as one of the identifying criteria when attempting to isolate Corynebacterium diphtheriae on Löffler's medium. Polyphosphate granules display the metachromatic effect, appearing red when stained with methylene blue.