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Although it could be used in other formats (e.g., Petri plates), Simmons' citrate agar is often used in slants or slopes in test tubes. One advantage of using slants over Petri dishes is access to oxygen, which is required by citrate metabolism. Simmons’ agar can be bought from suppliers as ready-made powders or slants.
These tubes are commonly sealed with a rubber stopper and often have a specific additive placed in the tube with the stopper color indicating the additive. For example, a blue-top tube is a 5 ml test tube containing sodium citrate as an anticoagulant, used to collect blood for coagulation and glucose-6-phosphate dehydrogenase testing. [5]
DNase agar is used to test whether a microbe can produce the exoenzyme deoxyribonuclease (DNase), which hydrolyzes DNA. Methyl green is used as an indicator in the growth medium because it is a cation that provides an opaqueness to a medium with the presence of negatively charged DNA strands.
The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate test. The lower case "i" is merely for "in" as the Citrate test requires coliform samples to be placed "in Citrate". These tests are useful in distinguishing members of ...
Acid-citrate-dextrose A (anticoagulant) Tissue typing, DNA studies, HIV cultures Pearlescent ("white") Separating gel and (K 2)EDTA: PCR for adenovirus, toxoplasma and HHV-6: Black Sodium Citrate: Paediatric ESR: QuantiFERON Grey, Green, Yellow, Purple QuantiFERON. 1. Grey (nil) tube 2. Green (TB1 antigen) tube 3. Yellow (TB2 antigen) tube 4 ...
Stab cultures are similar to agar plates, but are formed by solid agar in a test tube. Bacteria is introduced via an inoculation needle or a pipette tip being stabbed into the center of the agar. Bacteria grow in the punctured area. [ 11 ]
R2A agar, a nonspecific medium, imitates water, so is used for water analysis. Tryptic (trypticase) soy agar (TSA) is a general-purpose medium produced by enzymatic digestion of soybean meal and casein. It is frequently the base medium of other agar types; for example, blood agar plates are made by enriching TSA plates with blood.
This type of test is considered a diffusion test. [18] In agar and broth dilution methods, bacteria are placed in multiple small tubes with different concentrations of antibiotics. [14] Whether a bacterium is sensitive or not is determined by visual inspection or automatic optical methods, after a period of incubation. [5]