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Intrastrand and interstrand crosslinking of DNA. In genetics, crosslinking of DNA occurs when various exogenous or endogenous agents react with two nucleotides of DNA, forming a covalent linkage between them. This crosslink can occur within the same strand (intrastrand) or between opposite strands of double-stranded DNA (interstrand).
In vulcanization, sulfur is the cross-linking agent. Its introduction changes rubber to a more rigid, durable material associated with car and bike tires. This process is often called sulfur curing. In most cases, cross-linking is irreversible, and the resulting thermosetting material will degrade or burn if heated, without melting.
In 1984 John T. Lis and David Gilmour, at the time a graduate student in the Lis lab, used UV irradiation, a zero-length protein-nucleic acid crosslinking agent, to covalently cross-link proteins bound to DNA in living bacterial cells. Following lysis of cross-linked cells and immunoprecipitation of bacterial RNA polymerase, DNA associated with ...
Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order to identify RNA binding sites of proteins on a transcriptome-wide scale, thereby increasing our understanding of post-transcriptional regulatory networks.
A key feature of this two-step formaldehyde crosslinking reaction is that all the reactions are reversible, which is vital for chromatin capture. [1] [4] [15] [16] Crosslinking is a pivotal step of the chromatin capture workflow as the functional readout of the technique is the frequency at which two genomic regions are crosslinked to each ...
Spore photoproduct lyase (EC 4.1.99.14SP lyase, SPL, SplB, SplG) is a radical SAM enzyme that repairs DNA cross linking of thymine bases caused by UV-radiation.There are several types of thymine cross linking, but SPL specifically targets 5-thyminyl-5,6-dihydrothymine, which is also called spore photoproduct (SP).
Investigators got DNA from a knife sheath left at the crime scene and referenced testing on that sample in an affidavit supporting the arrest of Bryan Kohberger. DNA evidence likely key part of U ...
To assay for genotoxic molecules, researchers assay for DNA damage in cells exposed to the toxic substrates. This DNA damage can be in the form of single- and double-strand breaks, loss of excision repair, cross-linking, alkali-labile sites, point mutations, and structural and numerical chromosomal aberrations. [2]