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Therefore, the total number of reads generated in a single experiment is typically normalized by converting counts to fragments, reads, or counts per million mapped reads (FPM, RPM, or CPM). The difference between RPM and FPM was historically derived during the evolution from single-end sequencing of fragments to paired-end sequencing.
Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
Metric prefixes; Text Symbol Factor or; yotta Y 10 24: 1 000 000 000 000 000 000 000 000: zetta Z 10 21: 1 000 000 000 000 000 000 000: exa E 10 18: 1 000 000 000 000 000 000: peta P 10 15: 1 000 000 000 000 000: tera T
Genome size ranges (in base pairs) of various life forms. Genome size is the total amount of DNA contained within one copy of a single complete genome.It is typically measured in terms of mass in picograms (trillionths or 10 −12 of a gram, abbreviated pg) or less frequently in daltons, or as the total number of nucleotide base pairs, usually in megabases (millions of base pairs, abbreviated ...
For example, the algorithm QualComp [9] performs lossy compression with a rate (number of bits per quality value) specified by the user. Based on rate-distortion theory results, it allocates the number of bits so as to minimize the MSE (mean squared error) between the original (uncompressed) and the reconstructed (after compression) quality values.
The first American-made pocket-sized calculator, the Bowmar 901B (popularly termed The Bowmar Brain), measuring 5.2 by 3.0 by 1.5 inches (132 mm × 76 mm × 38 mm), came out in the Autumn of 1971, with four functions and an eight-digit red LED display, for US$240, while in August 1972 the four-function Sinclair Executive became the first ...
The HP-35, introduced on February 1, 1972, was Hewlett-Packard's first pocket calculator and the world's first handheld scientific calculator. [7] Like some of HP's desktop calculators it used RPN. Introduced at US$395, the HP-35 was available from 1972 to 1975.
The products of small numbers may be calculated by using the squares of integers; for example, to calculate 13 × 17, one can remark 15 is the mean of the two factors, and think of it as (15 − 2) × (15 + 2), i.e. 15 2 − 2 2.