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In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence. The term may refer to a polymorphism itself, as detected through the differing locations of ...
Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a molecular biology technique for profiling of microbial communities based on the position of a restriction site closest to a labelled end of an amplified gene. The method is based on digesting a mixture of PCR amplified variants of a single gene using one or more ...
When DNA analysis was first discovered, a process called Restriction Fragment Length Polymorphism (RFLP) was used to analyze DNA. However, RFLP was an inefficient process due to the fact that it used up large amounts of DNA which could not always be obtained from a crime scene. Modern day technology has evolved beyond RFLP.
Methods like Restriction fragment length polymorphism (RFLP), which was the first technique used for DNA analysis in forensic science, required high molecular weight DNA in the sample in order to get reliable data. High molecular weight DNA, however, is lacking in degraded samples, as the DNA is too fragmented to carry out RFLP accurately.
Terminal restriction fragment length polymorphism (T-RFLP) is a method that uses fluorescently-labeled DNA fragments to produce a community fingerprint. [2] [7] This section presents a brief explanation of T-RFLP in the specific context of community fingerprinting. For a more detailed explanation, refer to the T-RFLP article.
DNA paternity testing is the use of DNA profiles to determine whether an individual is the biological parent of another individual. Paternity testing can be especially important when the rights and duties of the father are in issue and a child's paternity is in doubt. Tests can also determine the likelihood of someone being a biological ...
The latter example is called restriction fragment length polymorphism (RFLP). [68] Artificial restriction enzymes created by linking the FokI DNA cleavage domain with an array of DNA binding proteins or zinc finger arrays, denoted zinc finger nucleases (ZFN), are a powerful tool for host genome editing due to their enhanced sequence specificity.
For restriction fragment length polymorphism analysis, these fragments are then visualized by gel electrophoresis. For RADseq, restriction fragments are ligated to an adapter that makes them readable by sequencing machines (not pictured), then fragments of a selected size range are sequenced using next-generation sequencing methods, aligned ...