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  2. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components.

  3. Genetic engineering techniques - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering_techniques

    In plants the DNA is often inserted using Agrobacterium-mediated recombination, [27] taking advantage of the Agrobacteriums T-DNA sequence that allows natural insertion of genetic material into plant cells. [28] Plant tissue are cut into small pieces and soaked in a fluid containing suspended Agrobacterium. The bacteria will attach to many of ...

  4. Boom method - Wikipedia

    en.wikipedia.org/wiki/Boom_method

    Boom method (aka Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid from a biological sample. This method is characterized by "absorbing the nucleic acids (NA) to the silica beads".

  5. Plant genetics - Wikipedia

    en.wikipedia.org/wiki/Plant_genetics

    An image of multiple chromosomes, taken from many cells. Plant genetics is the study of genes, genetic variation, and heredity specifically in plants. [1] [2] It is generally considered a field of biology and botany, but intersects frequently with many other life sciences and is strongly linked with the study of information systems.

  6. Genomic library - Wikipedia

    en.wikipedia.org/wiki/Genomic_library

    The vector containing the inserted fragments of genomic DNA can then be introduced into a host organism. [1] Below are the steps for creating a genomic library from a large genome. Extract and purify DNA. Digest the DNA with a restriction enzyme. This creates fragments that are similar in size, each containing one or more genes.

  7. DNA profiling - Wikipedia

    en.wikipedia.org/wiki/DNA_profiling

    DNA profiling (also called DNA fingerprinting and genetic fingerprinting) is the process of determining an individual's deoxyribonucleic acid characteristics. DNA analysis intended to identify a species, rather than an individual, is called DNA barcoding .

  8. Acid guanidinium thiocyanate-phenol-chloroform extraction

    en.wikipedia.org/wiki/Acid_guanidinium...

    RNA partitions in the aqueous phase, while proteins and DNA partition into the organic/interphase (left). The RNA is then precipitated in an alcohol (right). Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry and molecular biology.

  9. Archaeogenetics - Wikipedia

    en.wikipedia.org/wiki/Archaeogenetics

    However, silica itself is also a strong PCR inhibitor, so careful measures must be taken to ensure that silica is removed from the DNA after extraction. [24] The general process for extracting DNA using the silica-based method is outlined by the following: [21] Bone specimen is cleaned and the outer layer is scraped off; Sample is collected ...