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Epiboly in zebrafish is the first coordinated cell movement, beginning at the dome stage late in the blastula period and continuing throughout gastrulation. [3] At this point the zebrafish embryo contains three portions: an epithelial monolayer known as the enveloping layer (EVL), a yolk syncytial layer (YSL) which is a membrane-enclosed group of nuclei that lie on top of the yolk cell, and ...
Fish embryos go through a process called mid-blastula transition which is observed around the tenth cell division in some fish species. Once zygotic gene transcription starts, slow cell division begins and cell movements are observable. [4] During this time three cell populations become distinguished. The first population is the yolk syncytial ...
Zebrafish embryo 48 hours after fertilization. The arrows indicate four Rohon–Beard neurons. [1] Rohon–Beard cells are specialized neurons with mechanoreceptive properties. They occur during the embryonic stage of development and are found in the dorsal part of the spinal cord in fish and amphibians. [2]
The Zebrafish Information Network is an online biological database of information about the zebrafish (Danio rerio). The zebrafish is a widely used model organism for genetic , genomic , and developmental studies, and ZFIN provides an integrated interface for querying and displaying the large volume of data generated by this research. [ 2 ]
However, cells in the early embryo (less than 32 cells) are permeable to large molecules, [71] [72] allowing diffusion between cells. Guidelines for using Morpholinos in zebrafish describe appropriate control strategies. [73] Morpholinos are commonly microinjected in 500pL directly into 1–2 cell stage zebrafish embryos. The morpholino is able ...
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
PGC-like cells generated using this method can be transplanted into a gonad, where the differentiate, and are able to give viable gametes and offspring in vivo. [34] PGC-like cells can also be generated from naïve embryonic stem cells (ESCs) that are cultured for two days in the presence of FGF and Activin-A to adopt an epiblast-like state.
10. Halpern ME, Ho RK, Walker C, Kimmel CB 1993 Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation. Cell 75:99–111 11. Hug B, Walter V, Grunwald DJ 1997 tbx6, a Brachyury-related gene expressed by ventral mesendodermal precursors in the zebrafish embryo. Dev Biol 183:61–73 12. Goering LM et al. 2003.