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Zebrafish embryo 48 hours after fertilization. The arrows indicate four Rohon–Beard neurons. [1] Rohon–Beard cells are specialized neurons with mechanoreceptive properties. They occur during the embryonic stage of development and are found in the dorsal part of the spinal cord in fish and amphibians. [2]
In situ hybridizations of genes expressed in arteries (top) and veins (bottom) in zebrafish. Blue staining indicates presence of the gene mRNAs. Panels on the left are normal animals, while animals on the right are mutated in the Notch gene. Fish lacking Notch have fewer arteries and more veins at this point in developmental time.
This layer functions in protection by allowing the embryo to develop in a hypotonic solution so the cell will not burst. [5] Finally, the third set of blastomeres are the deep cells. These deep cells are located between the enveloping layer and the yolk syncytial layer and eventually give rise to the embryo proper. [1]
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, [2] it is a popular aquarium fish, frequently sold under the trade name zebra danio [3] (and thus often called a "tropical fish" although it is both tropical and subtropical).
Up to 18% of Morpholinos appear to induce nontarget-related phenotypes including cell death in the central nervous system and somite tissues of zebrafish embryos. [43] Most of these effects are due to activation of p53-mediated apoptosis and can be suppressed by co-injection of an anti-p53 Morpholino along with the experimental Morpholino.
Embryo of Zebrafish undergoing cleavage. The blastodisc, also called the germinal disc, is the embryo-forming part on the yolk of the egg of an animal that undergoes discoidal meroblastic cleavage. [1] Discoidal cleavage occurs in those animals with a large proportion of yolk in their eggs, and include insects, fish, reptiles and birds. [2]
VE-cadherin is indispensable for proper vascular development – there have been two transgenic mouse models of VE-cadherin deficiency, both embryonic lethal due to vascular defects. [ 10 ] [ 11 ] Further studies using one of these models revealed that although vasculogenesis occurred, nascent vessels collapsed or disassembled in the absence of ...