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This layer functions in protection by allowing the embryo to develop in a hypotonic solution so the cell will not burst. [5] Finally, the third set of blastomeres are the deep cells. These deep cells are located between the enveloping layer and the yolk syncytial layer and eventually give rise to the embryo proper. [1]
In situ hybridizations of genes expressed in arteries (top) and veins (bottom) in zebrafish. Blue staining indicates presence of the gene mRNAs. Panels on the left are normal animals, while animals on the right are mutated in the Notch gene. Fish lacking Notch have fewer arteries and more veins at this point in developmental time.
Epiboly in zebrafish is the first coordinated cell movement, beginning at the dome stage late in the blastula period and continuing throughout gastrulation. [3] At this point the zebrafish embryo contains three portions: an epithelial monolayer known as the enveloping layer (EVL), a yolk syncytial layer (YSL) which is a membrane-enclosed group of nuclei that lie on top of the yolk cell, and ...
The Zebrafish Information Network is an online biological database of information about the zebrafish (Danio rerio). The zebrafish is a widely used model organism for genetic , genomic , and developmental studies, and ZFIN provides an integrated interface for querying and displaying the large volume of data generated by this research. [ 2 ]
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, [2] it is a popular aquarium fish, frequently sold under the trade name zebra danio [3] (and thus often called a "tropical fish" although it is both tropical and subtropical).
Further, after clustering the zebrafish gene expression data into “transcription modules” reflecting each stage of development, they found multiple lines of evidence supporting the early conservation model (gene sequence, age, gene family size, and expression conservation) while only the analysis of gene regulatory regions supported the ...
Up to 18% of Morpholinos appear to induce nontarget-related phenotypes including cell death in the central nervous system and somite tissues of zebrafish embryos. [43] Most of these effects are due to activation of p53-mediated apoptosis and can be suppressed by co-injection of an anti-p53 Morpholino along with the experimental Morpholino.