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2. Folin–Ciocalteu reagent - Wikipedia

   en.wikipedia.org/wiki/Folin–Ciocalteu_reagent

   The Folin–Ciocâlteu reagent (FCR) or Folin's phenol reagent or Folin–Denis reagent, is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants, also called the gallic acid equivalence method (GAE). [1] It is named after Otto Folin, Vintilă Ciocâlteu, and Willey ...

3. Colorimetry (chemical method) - Wikipedia

   en.wikipedia.org/wiki/Colorimetry_(chemical_method)

   Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.

4. ATP test - Wikipedia

   en.wikipedia.org/wiki/ATP_test

   ATP is quantified by measuring the light produced through its reaction with the naturally occurring firefly enzyme luciferase using a luminometer. The amount of light produced is directly proportional to the amount of ATP present in the sample. ATP tests can be used to: Control biological treatment reactors; Guide biocide dosing programs

5. Colorimetric analysis - Wikipedia

   en.wikipedia.org/wiki/Colorimetric_analysis

   Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.

6. Lowry protein assay - Wikipedia

   en.wikipedia.org/wiki/Lowry_protein_assay

   The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is based on the reaction of Cu +, produced by the oxidation of peptide bonds, with Folin–Ciocalteu reagent (a mixture of phosphotungstic acid and phosphomolybdic acid in the Folin–Ciocalteu reaction).

7. 3,3',5,5'-Tetramethylbenzidine - Wikipedia

   en.wikipedia.org/wiki/3,3',5,5'-Tetramethylbenzidine

   3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays . [1] TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate.

8. FOX reagent - Wikipedia

   en.wikipedia.org/wiki/FOX_reagent

   The FOX assay relies on the oxidation of Fe 2+ to Fe 3+ in acidic conditions. [2] After this initial oxidation, the xylenol orange within the assay is also oxidized to produce the product that has the 560 nm absorbance via colorimetric determination. The method itself is practical because of its relative cheapness and because the FOX reagent is ...

9. 3'-Phosphoadenosine-5'-phosphosulfate - Wikipedia

   en.wikipedia.org/wiki/3'-Phosphoadenosine-5...

   Sulfate is not reduced directly but must be activated by the formation of APS or PAPS. These carriers of activated sulfate are produced by reaction with ATP. The first reaction is catalysed by ATP sulfurylase: SO 4 2− + ATP ⇌ APS + PP i. The conversion of APS to PAPS is catalysed by APS kinase: APS + ATP ⇌ PAPS + ADP