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Replica plating is a microbiological technique in which one or more secondary Petri plates containing different solid (agar-based) selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of microorganisms from a primary plate (or master dish), reproducing the ...
Replica plating is a technique that transfers colonies from one plate to another in the same spot as the last plate so the different media plates can be compared side by side. It is used to compare the growth of the same colonies on different plates of media to determine which environments the bacterial colony can or cannot grow in (this gives ...
Replica plating is another technique used to plate out cells on agar plates. These four techniques are the most common, but others are also possible. It is crucial to work in a sterile manner to prevent contamination on the agar plates. [1] Plating is thus often done in a laminar flow cabinet or on the working bench next to a bunsen burner. [2]
The results of Luria and Delbrück were confirmed in more graphical, but less quantitative, way by Newcombe. Newcombe incubated bacteria in a Petri dish for a few hours, then replica plated it onto two new Petri dishes treated with phage. The first plate was left unspread, and the second plate was then respread, that is, bacterial cells were ...
Esther Miriam Zimmer Lederberg (December 18, 1922 – November 11, 2006) was an American microbiologist and a pioneer of bacterial genetics.She discovered the bacterial virus lambda phage and the bacterial fertility factor F, devised the first implementation of replica plating, and furthered the understanding of the transfer of genes between bacteria by specialized transduction.
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A schematic representation of the pBR322 vector with restriction sites indicated in blue. pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. ...
The replicas may be imaged in the light microscope or coated with heavy metals, the replicating film melted away, and the heavy metal replica imaged in a Transmission Electron Microscope (TEM). The same materials, cellulose acetate films , are used for creating replicas of biological materials such as bacteria.