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Gel permeation chromatography (GPC) [1] is a type of size-exclusion chromatography (SEC), that separates high molecular weight or colloidal analytes on the basis of size or diameter, typically in organic solvents. The technique is often used for the analysis of polymers.
Gel permeation chromatography is often used in combination with multi-angle light scattering (MALS), Low-angle laser light scattering (LALLS) and/or viscometry for an absolute determination (i.e., independent of the chromatographic separation details) of the molecular weight distribution as well as the branching ratio and degree of long chain ...
The main application of size-exclusion chromatography is the fractionation of proteins and other water-soluble polymers, while gel permeation chromatography is used to analyze the molecular weight distribution of organic-soluble polymers.
Size-exclusion chromatography (SEC) is also known as gel permeation chromatography (GPC) or gel filtration chromatography and separates molecules according to their size (or more accurately according to their hydrodynamic diameter or hydrodynamic volume). Smaller molecules are able to enter the pores of the media and, therefore, molecules are ...
The most common technique for measuring molecular mass used in modern times is a variant of high-pressure liquid chromatography (HPLC) known by the interchangeable terms of size exclusion chromatography (SEC) and gel permeation chromatography (GPC).
Gel permeation chromatography-IR spectroscopy (GPC-IR) H. High performance liquid chromatography ... Pyrolysis gas chromatography mass spectrometry (PY-GC-MS)
Gas chromatography; Gel permeation chromatography; Thin-layer chromatography; Van Deemter equation; Phase separations. This section contains 22 articles.
Determination of the Low Molecular Weight Content of a Polymer Using Gel Permeation Chromatography 120: Solution/Extraction Behaviour of Polymers in Water 121: Estimation of the adsorption coefficient (K OC) on Soil and on sewage sludge using High Performance Liquid Chromatography (HPLC) 122: Determination of pH, Acidity and Alkalinity: 123
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