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  2. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.

  3. Fluorescence microscope - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_microscope

    Fluorescence microscopy requires intense, near-monochromatic, illumination which some widespread light sources, like halogen lamps cannot provide. [4] Four main types of light source are used, including xenon arc lamps or mercury-vapor lamps with an excitation filter, lasers, supercontinuum sources, and high-power LEDs.

  4. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    This shape is called the point spread function (PSF) of the microscope imaging system. Since any fluorescence image is made up of a large number of such small fluorescent light sources, the image is said to be "convolved by the point spread function". The mathematically modeled PSF of a terahertz laser pulsed imaging system is shown on the right.

  5. Point spread function - Wikipedia

    en.wikipedia.org/wiki/Point_spread_function

    An example of an experimentally derived point spread function from a confocal microscope using a 63x 1.4NA oil objective. It was generated using Huygens Professional deconvolution software. Shown are views in xz, xy, yz and a 3D representation. In microscopy, experimental determination of PSF requires sub-resolution (point-like) radiating sources.

  6. Bright-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Bright-field_microscopy

    The light path of a bright-field microscope is extremely simple; no additional components are required beyond the normal light-microscope setup. The light path begins at the illuminator or the light source on the base of the microscope. Often a halogen lamp is used. The light travels through the objective lens into the ocular lens, through ...

  7. Köhler illumination - Wikipedia

    en.wikipedia.org/wiki/Köhler_illumination

    Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.

  8. Light sheet fluorescence microscopy - Wikipedia

    en.wikipedia.org/wiki/Light_sheet_fluorescence...

    on YouTube: The linked video shows the development of a fruit fly embryo, which was recorded during 20 hours. Two projections of the full 3D dataset are shown. The mesoSPIM Initiative. Open-source light-sheet microscopes for imaging cleared tissue.

  9. Stereo microscope - Wikipedia

    en.wikipedia.org/wiki/Stereo_microscope

    The stereo, stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it. The instrument uses two separate optical paths with two objectives and eyepieces to provide slightly ...

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