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  2. Anti-thymocyte globulin - Wikipedia

    en.wikipedia.org/wiki/Anti-thymocyte_globulin

    Anti-thymocyte globulin (ATG) is an infusion of horse or rabbit-derived antibodies against human T cells and their precursors , which is used in the prevention and treatment of acute rejection in organ transplantation and therapy of aplastic anemia due to bone marrow insufficiency.

  3. FLAG-tag - Wikipedia

    en.wikipedia.org/wiki/FLAG-tag

    Unlike some other tags (e.g. myc, HA), where a monoclonal antibody was first isolated against an existing protein, then the epitope was characterized and used as a tag, the FLAG epitope was an idealized, artificial design, to which monoclonal antibodies were raised. The FLAG-tag's sequence was optimized for compatibility with proteins it is ...

  4. Rabbit hybridoma - Wikipedia

    en.wikipedia.org/wiki/Rabbit_hybridoma

    The rabbit formed a myeloma-like tumor, allowing the isolation of a plasmacytoma cell line, named 240E-1. Fusion of 240E-1 cells with rabbit lymphocytes produced hybridomas that secreted rabbit monoclonal antibodies in a consistent manner. [4] However, like the early mouse myeloma lines developed in the 1970s, stability was a concern.

  5. Anti-immunoglobulin - Wikipedia

    en.wikipedia.org/wiki/Anti-immunoglobulin

    This is a recombinant monoclonal antibody to IgG. When using ELISA, the anti-antibody also recognizes rhesus macaque IgG1, cynomolgus monkey IgG1, and cynomolgus monkey IgG4. [10] Anti-IgG3 [NH3/15.8] This is a recombinant monoclonal antibody to IgG3. Anti-IgG3 [NH3/15.8] is most commonly used in human IgG blood transfusion serology testing. [11]

  6. Polyclonal antibodies - Wikipedia

    en.wikipedia.org/wiki/Polyclonal_antibodies

    However, polyclonal antibodies in mice can be collected from ascites fluid using any one of a number of ascites producing methodologies. When using rabbits, young adult animals (2.5–3.0 kg or 5.5–6.5 lb) should be used for primary immunization because of the vigorous antibody response.

  7. Myc-tag - Wikipedia

    en.wikipedia.org/wiki/Myc-tag

    A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism.

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