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Bromothymol is used in obstetrics for detecting premature rupture of membranes. [11] Amniotic fluid typically has a pH > 7.2, bromothymol will therefore turn blue when brought in contact with fluid leaking from the amnion. As vaginal pH normally is acidic, the blue color indicates the presence of amniotic fluid.
Paper form: It is a strip of coloured paper which changes colour to red if the solution is acidic and to blue, if the solution is basic. The strip can be placed directly onto a surface of a wet substance or a few drops of the solution can be dropped onto the universal indicator using dropping equipment.
Conversely, if a 10-fold excess of the acid occurs with respect to the base, the ratio is 1:10 and the pH is pK a − 1 or pK b − 1. For optimal accuracy, the color difference between the two species should be as clear as possible, and the narrower the pH range of the color change the better.
The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change. Sometimes, it is possible to detect growth on the Simmons' citrate agar without the accompanying color change to blue. This should be scored as a negative for the citrate use test. [12]
Bromothymol blue is the indicator used in the agar, it changes to yellow in case of acid production during fermentation of lactose or changes to deep blue in case of alkalinization. Lactose-positive bacteria build yellow colonies. Bacteria which decarboxylate L-cystine cause an alkaline reaction and build deep blue colonies. [1]
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Phenolphthalein's common use is as an indicator in acid-base titrations. It also serves as a component of universal indicator, together with methyl red, bromothymol blue, and thymol blue. [3] Phenolphthalein adopts different forms in aqueous solution depending on the pH of the solution.
RDS Delivery Service president and CEO Larry Zogby claimed congestion pricing will cost his business, run out of Long Island City in Queens to all New York boroughs, more than a million dollars ...
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