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  2. CRISPR - Wikipedia

    en.wikipedia.org/wiki/CRISPR

    CRISPR-Cas immunity is a natural process of bacteria and archaea. [103] CRISPR-Cas prevents bacteriophage infection, conjugation and natural transformation by degrading foreign nucleic acids that enter the cell. [38]

  3. CRISPR gene editing - Wikipedia

    en.wikipedia.org/wiki/CRISPR_gene_editing

    CRISPR gene editing (CRISPR, pronounced / ˈkrɪspər / "crisper", refers to " c lustered r egularly i nterspaced s hort p alindromic r epeats") is a genetic engineering technique in molecular biology by which the genomes of living organisms may be modified.

  4. CRISPR activation - Wikipedia

    en.wikipedia.org/wiki/CRISPR_activation

    CRISPR activation (CRISPRa) is a gene regulation technique that utilizes an engineered form of the CRISPR-Cas9 system to enhance the expression of specific genes without altering the underlying DNA sequence. Unlike traditional CRISPR-Cas9, which introduces double-strand breaks to edit genes, CRISPRa employs a modified, catalytically inactive ...

  5. CRISPR interference - Wikipedia

    en.wikipedia.org/wiki/CRISPR_interference

    CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression of gene expression in prokaryotic and eukaryotic cells. [1] It was first developed by Stanley Qi and colleagues in the laboratories of Wendell Lim , Adam Arkin, Jonathan Weissman , and Jennifer Doudna . [ 2 ]

  6. CRISPR RNA - Wikipedia

    en.wikipedia.org/wiki/CRISPR_RNA

    CRISPR RNA or crRNA is a RNA transcript from the CRISPR locus. [1] CRISPR-Cas (clustered, regularly interspaced short palindromic repeats - CRISPR associated systems) is an adaptive immune system found in bacteria and archaea to protect against mobile genetic elements , like viruses , plasmids , and transposons . [ 2 ]

  7. Site-directed mutagenesis - Wikipedia

    en.wikipedia.org/wiki/Site-directed_mutagenesis

    Site-directed mutagenesis is a molecular biology method that is used to make specific and intentional mutating changes to the DNA sequence of a gene and any gene products. Also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, it is used for investigating the structure and biological activity of DNA, RNA, and protein ...

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