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The equipment required is a colorimeter, some cuvettes and a suitable color reagent. The process may be automated, e.g. by the use of an AutoAnalyzer or by flow injection analysis. Recently, colorimetric analyses developed for colorimeters have been adapted for use with plate readers to speed up analysis and reduce the waste stream. [1]
Colorimetry is "the science and technology used to quantify and describe physically the human color perception". [1] It is similar to spectrophotometry, but is distinguished by its interest in reducing spectra to the physical correlates of color perception, most often the CIE 1931 XYZ color space tristimulus values and related quantities.
Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
CIELAB D50 is available in ICC profiles as a profile connection space named "Lab color space". [2] CIELAB (any white point) is a supported color space in TIFF image files. [17] CIELAB (any white point) is available in PDF documents, where it is called the "Lab color space". [18] [19] CIELAB is an option in Digital Color Meter on macOS described ...
"ColorChecker RGB Summaries, Spreadsheets and Lab TIFF File". brucelindbloom.com. A page showing RGB values for color patches in various RGB color spaces, based on the applet described above, and a set of Excel spreadsheets for comparing these numbers to those in a digital camera or scanner image of the ColorChecker.
A color in the RGB color model is described by indicating how much of each of the red, green, and blue is included. The color is expressed as an RGB triplet (r,g,b), each component of which can vary from zero to a defined maximum value. If all the components are at zero the result is black; if all are at maximum, the result is the brightest ...
It is able to store a wider range of color values than sRGB. The Wide Gamut color space is an expanded version of the Adobe RGB color space, developed in 1998. As a comparison, the Adobe Wide Gamut RGB color space encompasses 77.6% of the visible colors specified by the Lab color space, whilst the standard Adobe RGB color space covers just 50.6%.