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The mechanism of light creation is through a suborbital photophore that utilizes gland cells which produce exergonic chemical reactions that produce light with a longer, red wavelength. [74] The dragonfish species which produce the red light also produce blue light in photophore on the dorsal area. [ 74 ]
In biochemical spectroscopy, information can be gathered about biological tissue by absorption and light scattering techniques. Light scattering spectroscopy is a type of reflectance spectroscopy that determines tissue structures by examining elastic scattering. [10] In such a case, it is the tissue that acts as a diffraction or dispersion ...
They are non-thermal in origin, and the emission of biophotons is technically a type of bioluminescence, though the term "bioluminescence" is generally reserved for higher luminance systems (typically with emitted light visible to the naked eye, using biochemical means such as luciferin/luciferase).
Spectrophotometry is an important technique used in many biochemical experiments that involve DNA, RNA, and protein isolation, enzyme kinetics and biochemical analyses. [16] Since samples in these applications are not readily available in large quantities, they are especially suited to be analyzed in this non-destructive technique.
Photosynthetically active radiation (PAR) spans the visible light portion of the electromagnetic spectrum from 400 to 700 nanometers. Photosynthetically active radiation (PAR) designates the spectral range (wave band) of solar radiation from 400 to 700 nanometers that photosynthetic organisms are able to use in the process of photosynthesis.
For bacterial bio-luminescence specifically, the biochemical reaction involves the oxidation of an aliphatic aldehyde by a reduced flavin mononucleotide. [14] The products of this oxidation reaction include an oxidized flavin mononucleotide, a fatty acid chain, and energy in the form of a blue-green visible light.
Molecules have various states referred to as energy levels.Fluorescence spectroscopy is primarily concerned with electronic and vibrational states. Generally, the species being examined has a ground electronic state (a low energy state) of interest, and an excited electronic state of higher energy.
Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay , that consists of a simple prepare and read method, without the requirement of separation or washing steps.