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Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
The original interface between capillary zone electrophoresis and mass spectrometry was developed in 1987 [9] by Richard D. Smith and coworkers at Pacific Northwest National Laboratory, and who also later were involved in development of interfaces with other CE variants, including capillary isotachophoresis and capillary isoelectric focusing.
[1] [2] Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube , which is filled with the analyte.
The apparatus consists of a vial with a conical bottom, a grounded platinum electrode, a capillary to inject the aqueous solution, and an adjustable gold anode with a circular bottom that contacts the entire organic phase. EE is also often performed in a capillary electrophoresis capillary. This is referred to as capillary electroextraction, or ...
When all of the TE ions are dissolved, the focusing process ceases and the analytes are separated according to the principles of zone electrophoresis. tITP is nowadays more widespread than conventional ITP because it is easily implemented in capillary electrophoresis (CE) separations as a preconcentration step, making CE more sensitive while ...
Different KCE methods are designed by varying initial and boundary conditions – the way interacting molecules enter and exit the capillary. Several KCE methods were described: non-equilibrium capillary electrophoresis of the equilibrium mixtures (NECEEM), [2] sweeping capillary electrophoresis (SweepCE), [3] and plug-plug KCE (ppKCE). [4]
The PCR products are quantified, typically by (capillary) electrophoresis. Each probe pair consists of two oligonucleotides, with sequence that recognizes adjacent sites of the target DNA, a PCR priming site, and optionally a "stuffer" to give the PCR product a unique length when compared to other probe pairs in the MLPA assay.
The DNA fragments that result are then separated and detected using electrophoresis. There are two common methods of separation and detection, capillary electrophoresis (CE) and gel electrophoresis. Each STR is polymorphic, but the number of alleles is very small. Typically each STR allele will be shared by around 5 - 20% of individuals.