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In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...
URA3 is often used in yeast research as a "marker gene", that is, a gene to label chromosomes or plasmids. URA3 encodes Orotidine 5'-phosphate decarboxylase (ODCase) , which is an enzyme that catalyzes one reaction in the synthesis of pyrimidine ribonucleotides (a component of RNA ).
In the peer-reviewed literature report, experimental results on function and interaction of yeast genes are extracted by high-quality manual curation and integrated within a well-developed database. The data are combined with quality high-throughput results and posted on Locus Summary pages which is a powerful query engine and rich genome browser.
Y187 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening.The strain has been sold commercially by Clontech [1] since at least 2000 and is used as a partner with strain AH109 in mating assays.
In the 1990s, few genes were known and analysed by scientists until the first genomic analysis was performed by a team of the Pasteur Institute of Paris. [2] The genome Kluyveromyces lactis was explored by sequencing 588 short tags from two random genomic libraries (random sequenced tags, or RSTs). 296 K. lactis genes were identified of which 292 were new.
Nutritional yeast is a deactivated version of the same yeast used to make bread rise, says Courtney Pelitera, M.S., R.D., C.N.S.C., registered dietitian and owner of Devour Your Life. “The yeast ...
Mammalian cells, yeast, and other eukaryotes acquire resistance to geneticin (= G418, an aminoglycoside antibiotic similar to kanamycin) when transformed with a kanMX marker. In yeast, the kanMX marker avoids the requirement of auxotrophic markers. In addition, the kanMX marker renders E. coli resistant to kanamycin.
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