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Solid-phase extraction (SPE) [1] is a solid-liquid extractive technique, by which compounds that are dissolved or suspended in a liquid mixture are separated, isolated or purified, from other compounds in this mixture, according to their physical and chemical properties. Analytical laboratories use solid phase extraction to concentrate and ...
Solid-phase microextraction sampling. Solid phase microextraction, or SPME, is a solid phase extraction sampling technique that involves the use of a fiber coated with an extracting phase, that can be a liquid or a solid (), [1] which extracts different kinds of analytes (including both volatile and non-volatile) from different kinds of media, that can be in liquid or gas phase. [2]
Laboratory-scale liquid-liquid extraction. Photograph of a separatory funnel in a laboratory scale extraction of 2 immiscible liquids: liquids are a diethyl ether upper phase, and a lower aqueous phase. Soxhlet extractor. Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix. The distribution ...
The reagents used depend on the type of sample to be analyzed. Following this, the sample is put through a dispersive solid phase extraction cleanup prior to analysis by gas-liquid chromatography or liquid-liquid chromatography. Samples prepared using the QuEChERS method can be processed more quickly using a homogenization instrument.
Boom method (aka Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid from a biological sample. This method is characterized by "absorbing the nucleic acids (NA) to the silica beads".
Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions.
However, much like the Soxhlet extraction technique, this method also is very time-consuming. [48] Solid-phase template As described above, one benefit of immobilising the template molecule on a solid support such as glass beads is the easy removal of the MIPs from the template. Following a cold wash to remove unreacted monomers and low ...
Unfortunately, Chelex extraction does not yield as much quantity and the DNA yielded is single-stranded, which means it can only be used for PCR-based analyses and not for RFLP. [9] Solid phase extraction such as using a spin-column-based extraction method takes advantage of the fact that DNA binds to silica.
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