Search results
Results from the WOW.Com Content Network
Individual clones from genomic libraries can be sheared into smaller fragments, usually 500bp to 1000bp, which are more manageable for sequencing. [4] Once a clone from a genomic library is sequenced, the sequence can be used to screen the library for other clones containing inserts which overlap with the sequenced clone.
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism.
A genomic library is a set of clones that together represents the entire genome of a given organism. The number of clones that constitute a genomic library depends on (1) the size of the genome in question and (2) the insert size tolerated by the particular cloning vector system. For most practical purposes, the tissue source of the genomic DNA ...
Computational genomics refers to the use of computational and statistical analysis to decipher biology from genome sequences and related data, [1] including both DNA and RNA sequence as well as other "post-genomic" data (i.e., experimental data obtained with technologies that require the genome sequence, such as genomic DNA microarrays).
Microarrays require some genomic knowledge from the organism of interest, for example, in the form of an annotated genome sequence, or a library of ESTs that can be used to generate the probes for the array.
Functional genomics make use of the vast data generated by genomic and transcriptomic projects (such as genome sequencing projects and RNA sequencing). Functional genomics focuses on the dynamic aspects such as gene transcription , translation , regulation of gene expression and protein–protein interactions , as opposed to the static aspects ...
Gene has been implemented at NCBI to characterize and organize the information about genes. It serves as a major node in the nexus of the genomic map, expression, sequence, protein function, structure, and homology data. A unique GeneID is assigned to each gene record that can be followed through revision cycles.
Alternatively, a jumping library can be used with NGS for the mapping of structural variation and scaffolding of de novo assemblies. [8] This figure is a schematic representation of one of the most recently used methods for creating jumping libraries. Jumping libraries can be categorized according to the length of the incorporated DNA fragments.